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Scientist I

Cambridge

30.00-30.00

Contract

Job description:Develops and executes molecular and cellular assays and methodologies to support target validation projects as well as drug discovery programs.Develops, optimizes and implements functional genomic assays and potentially screen assays for novel target discovery.Molecular cloning (recombinant DNA cloning, PCR, plasmid preparation, DNA sequencing analysis, site directed mutagenesis).Performs Genome editing, using CRISPR/Cas9 system to KO or KI gene in mammalian cells.Designs and performs siRNA transfection; inducible shRNA lentivirus infection and rescue with shRNA resistant cDNA.Performs Generation Lentivirus vector, packaging virus, virus titer, transduction mammalian cells and establishes stable transgenic line. 

Posted 13 days ago

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